2 replies to this topic

[saraarasus]

Hi,
I want to do dot blot. Has any one know if I need to we the membrane in transfer buffer and methanot or I can do it right away.

Many thanks,
Sara

[dtimm]

This website should answer your question.

http://www.millipore...ech1/tn1004enus

[bob1]

If you are using PVDF you will need to wet the membrane beforehand as it is hydrophobic until placed in an appropriate solvent (methanol)