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Dot blot


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#1 saraarasus

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Posted 05 May 2009 - 11:53 PM

Hi,
I want to do dot blot. Has any one know if I need to we the membrane in transfer buffer and methanot or I can do it right away.

Many thanks,
Sara

#2 dtimm

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Posted 06 May 2009 - 10:30 AM

This website should answer your question.

http://www.millipore...ech1/tn1004enus

#3 bob1

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Posted 06 May 2009 - 04:33 PM

If you are using PVDF you will need to wet the membrane beforehand as it is hydrophobic until placed in an appropriate solvent (methanol)




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