I have managed to label pneumococcal polysaccharide with Alexa 488 using a CDAP activation method. The labeled sample was then cleaned up using a PD10 column.
We're using the labeled PPS to sort out populations of PPS specific B cells. However through a series of unsuccessful inhibition experiments we know that this population of labeled PPS is not 100% labeled and there is still some unlabeled PPS.
Anyone have any ideas on how to separate these two populations?
We've already looked into HPLC. Our university does not have this equipment and it will cost too much time (4-6 weeks) and money ($2500).
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