Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

western blot stripping buffer


  • Please log in to reply
2 replies to this topic

#1 apple9

apple9

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 04 May 2009 - 08:02 AM

Could anyone recommend a stripping buffer for re-bloting western blot membranes? Thanks.

#2 Guillermina

Guillermina

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 04 May 2009 - 11:51 AM

I use the following:

100mM 2-mercapto ethanol
2% SDS
62.5 mM Tris HCl PH6.7

#3 newarray

newarray

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 04 May 2009 - 12:59 PM

If it is PVDF membrane i use 0.2 N NaoH.
Its so convinient and time also less.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.