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Na2CO3 addition instead of NaHCO3 to RPMI/PS


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#1 Danina

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Posted 03 May 2009 - 11:49 PM

Dear all,

a week ago, I accidentally added 2 g of Na2CO3 to my PRMI/Pennicilin Streptomycin stock instead of adding 2 g of NaHCO3. I wonder if anyone had this mistake done before and what happen to your cells? I am maintaining LNCaP and DU145. Morphologically, they look 'normal' as normal as cancer cells can be, but I am worried whether they will have mutation etc that will cause problems in subsequent experiements.

Thanks for your help :)

Dani

#2 gfischer

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Posted 04 May 2009 - 10:33 AM

Dear all,

a week ago, I accidentally added 2 g of Na2CO3 to my PRMI/Pennicilin Streptomycin stock instead of adding 2 g of NaHCO3. I wonder if anyone had this mistake done before and what happen to your cells? I am maintaining LNCaP and DU145. Morphologically, they look 'normal' as normal as cancer cells can be, but I am worried whether they will have mutation etc that will cause problems in subsequent experiements.

Thanks for your help :)

Dani


The purpose of NaHCO3 is generally just a pH buffer, and since Na2CO3 is also a salt of an acid and a base, it should also work. That said, I would try to get them in correctly formulated medium as soon as possible, since the equilibria are different. This probably won't affect the cells, but it may have implications for any other manipulations you do, like treatments with drugs.
Above all things, if kindness is your king,
then heaven will be yours, before you meet your end

#3 Danina

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Posted 04 May 2009 - 10:58 PM

Hey gfischer,

thanks for the info. Yeap, I have put them back on NaHCO3 and they are looking good :P

Cheers,
Dani




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