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Thermal stability of H202


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#1 rhythmgenes

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Posted 03 May 2009 - 11:11 PM

Hi everyone,

I just needed to know the best way to add hydrogen peroxide in an agar. I understand H202 can't be autoclaved. So I need to add it after the agar cools down. But everytime I do it, I am not sure if I am adding it at the right temperature so as not to degrade the H202. Anyone know if I can add H202 when the temperature is around 70 degree celsius which is when I usually add it. If I go to ard 60 degree celsius, then I have to be very quick otherwise the agar solidifies. Any suggestions would be appreciable.

Regards,
rigien

#2 T C

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Posted 04 May 2009 - 11:02 PM

Don't have much experience with this but why can't you just spread it on top of agar when it cools down?

Hi everyone,

I just needed to know the best way to add hydrogen peroxide in an agar. I understand H202 can't be autoclaved. So I need to add it after the agar cools down. But everytime I do it, I am not sure if I am adding it at the right temperature so as not to degrade the H202. Anyone know if I can add H202 when the temperature is around 70 degree celsius which is when I usually add it. If I go to ard 60 degree celsius, then I have to be very quick otherwise the agar solidifies. Any suggestions would be appreciable.

Regards,
rigien






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