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pqe30


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#1 atul21985

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Posted 03 May 2009 - 07:39 AM

i have cloned my gene into pqe30 vector,clones are confirmed but now i am not able to transform it into bl21de3,c41,rosetta,plys or any of these, comp cells are good control is working can anyone help me

#2 T C

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Posted 03 May 2009 - 09:20 PM

Are you using the right antibiotic....sorry for this question but it happens sometimes :)

i have cloned my gene into pqe30 vector,clones are confirmed but now i am not able to transform it into bl21de3,c41,rosetta,plys or any of these, comp cells are good control is working can anyone help me






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