Posted 01 May 2009 - 04:02 AM
I need to detach a biofilm from a surface which will then be processed to extract DNA. I am just wondering if sonication will be enought to remove all attached bacteria. The surface the biofilm will be attached to is a thin clay tablet. Any ideas?
Posted 02 May 2009 - 05:04 AM
Why not remove it with a tool - like a sterile spatula?
I believe that removing it with a sterile spatula would still perhaps leave bacteria attached to the surface. In very thin biofilms, on the other hand, I guess it would not work (¿?).
Posted 02 May 2009 - 05:30 AM
Posted 02 May 2009 - 06:47 AM
thanks for your reply. should the phenol/chloroform extraction of the biofilm interfere with subsequent DNA extraction from it using e.g., a DNA isolation kit?
I would include a sample of the clay tablet in the lysis reaction. Chemistry will remove things that mechanical disruption will not. A phenol/chloroform extraction would likely remove most anything organic.
Posted 06 May 2009 - 06:56 PM
Posted 12 March 2010 - 08:26 AM