2 replies to this topic

[ni_labgirl]

Hi all,

Just a quick question- I have been working on mtt assays to test the sensitivity of 2 cell lines t drugs. I have been using a range of concentrations as determined by literature I have read, however my supervisor has asked me how relevant these doses are to what is used clinically? I have no idea - any idea where would be the best place to start looking? The drug im using presently is cisplatin.

thanks

[SamOH]

ni_labgirl, on May 1 2009, 03:33 AM, said:

Hi all,

Just a quick question- I have been working on mtt assays to test the sensitivity of 2 cell lines t drugs. I have been using a range of concentrations as determined by literature I have read, however my supervisor has asked me how relevant these doses are to what is used clinically? I have no idea - any idea where would be the best place to start looking? The drug im using presently is cisplatin.

thanks

The package insert may be a good place to start. Also, I'm sure there are a lot of papers looking at the pharmacokinetics of the drug in patients. I would look for plasma concentrations achieved in patients given the clinically relevant doses. Although the important drug concentration is that which the tumor "sees" in vivo (i.e. intratumoral concentrations), plasma (i.e. blood) concentrations are the rough estimate that people often use to assess whether the in vitro dose they are using is in the ballpark of in vivo-relevant doses.

Good luck!
Sam

[bob1]

Yep, heaps and heaps of papers on cisplatin and relevant doses in patients and in cell culture, as these are two quite different things.