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Pancreas RNA extraction


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2 replies to this topic

#1 wuxx0153

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Posted 30 April 2009 - 07:08 AM

We are going to have RNA extract from pancreas, the major source of RNase production, and the first try was not looking very good.

Is there anyone have improved RNA extraction protocol from this difficult tissue?

#2 rustyshackleford

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Posted 30 April 2009 - 06:27 PM

Hello,

I've had success using the protocol outlined in the following paper:

Li D, Ren W, Wang X, Wang F, Gao Y, Ning Q, Han Y, Song T, Lu S. A Modified Method using TRIzol Reagent and Liquid Nitrogen Produces High-Quality RNA from Rat Pancreas. Appl Biochem Biotechnol. 2008 Oct 18. [Epub ahead of print].

Best of luck! ;)

#3 wuxx0153

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Posted 01 May 2009 - 07:52 AM

Hello,

I've had success using the protocol outlined in the following paper:

Li D, Ren W, Wang X, Wang F, Gao Y, Ning Q, Han Y, Song T, Lu S. A Modified Method using TRIzol Reagent and Liquid Nitrogen Produces High-Quality RNA from Rat Pancreas. Appl Biochem Biotechnol. 2008 Oct 18. [Epub ahead of print].

Best of luck! :huh:

Thanks a lot

I know what went wrong now, I though 100 mg/ml TRIzol was good enough to protect RNAs.




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