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[sssss]

hiiiiiiiiiiiiiiiii,
how to transfer protein from a NATIVE PAGE gel to membrane.
Is ther any protocol??????????/

[klinmed]

sssss, on Apr 30 2009, 12:12 PM, said:

hiiiiiiiiiiiiiiiii,
how to transfer protein from a NATIVE PAGE gel to membrane.
Is ther any protocol??????????/

Transfer as if using denaturing gels. Tris/glycine for PVDF membranes or Tris/glycine/20% methanol for nitrocellulose.
For a lot of antigenic epitopes, the immobilisation to a membrane is more denaturing than using a transfer buffer containing methanol.

Hope this helps.