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#1 sssss

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Posted 30 April 2009 - 02:12 AM

hiiiiiiiiiiiiiiiii,
how to transfer protein from a NATIVE PAGE gel to membrane.
Is ther any protocol??????????/

#2 klinmed

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Posted 30 April 2009 - 12:18 PM

hiiiiiiiiiiiiiiiii,
how to transfer protein from a NATIVE PAGE gel to membrane.
Is ther any protocol??????????/

Transfer as if using denaturing gels. Tris/glycine for PVDF membranes or Tris/glycine/20% methanol for nitrocellulose.
For a lot of antigenic epitopes, the immobilisation to a membrane is more denaturing than using a transfer buffer containing methanol.

Hope this helps.




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