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A couple of questions about Cell Factories


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#1 jonfraz

jonfraz

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Posted 30 April 2009 - 01:01 AM

I'm an engineer doing some design work with cell factories (primarily the 10 layer varients) and I was hoping to get some info from people who've actually used them for cell culture!

I have a couple of main questions, however any reviews or input would be much appreciated.

1. Does anyone know the max filling rate (using a peristaltic pump) for a cell factory?
I've spoken to nunc technical support on this but they don't have any figures, they just warned me about pressure build up.

2. Is it possible to "top up" a cell factory with medium without emptying it first, or will this risk contamination?
The main issue here is whether you can seed the cell factory with a high concentration of cells but then dilute it in situ rather than beforehand.

I hope some people can help a poor engineer!

Thanks




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