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[mrflibbles0412]

All hail to the scholars in this virtual room.  I’m in the company of pure genius.  Here’s a basic tissue culture question that I’m confident you guys will knock right out of the ball park.   I just want to grow a few hybridomas at the same density, 1 X 10 e 5, even though they start off at different densities.  In other words, I have roughly 750, 000 total cells or 25 mls with a cell count of 3.0 X 10 e 4 cells/ml.  Another flask has 20,000 cells/ml in a volume of 50 mls for 1 million cells total.  My preference is to grow them in a T25 flask but the vessel doesn’t matter much---T50, T75.    So, should I divide the total number of cells by the desired density?  I could add 7.5 mls cells to 50 mls media for the first flask and 10 mls cells to 50 mls media for the second flask.  Right?

[bob1]

V=N/C (volume=number divided by concentration) is your friend here, it doesn't just apply to chemistry, any solution will do!