cell culture best practices
Posted 29 April 2009 - 10:48 AM
I thought that it was always a bad practice to culture virus transformed (immortalized) cell line(s) and non-transformed cells in the same culture laboratory or, at least in the same incubator. This is because of the possibity of cross contamination of the virus from the transformed cells to the non-transformed cell line(s). This contamination might lead to a transformation of the previous normal replicating
Because recombinant methods are evolving, I suppose it is possible to construct a viral vector that cannot be unintentionally transferred
to another cell line. But, I do not think that this is yet a normal practice.
Any comments would be appreciated.
Posted 29 April 2009 - 04:48 PM
The vast majority of cells that are virally transformed do not shed any virus, and are surprisingly common. Common ones you might have come across include HeLa, HepG2, Hep3B, and HEK293. Most cervical cancer lines are transformed by HPV, and most liver ones by hepatitis. There is some evidence that viruses mediate about 15% of the total cancers incidence worldwide.
It is fine to culture cells that do not shed live virus in the same incubator as other cell lines, ones that do shed should of course be kept in separate incubators and with appropriate conditions to ensure that you don't infect your other cells.
Edited to add: you should of course quarantine any cell lines that you get into the lab to ensure that they aren't infected with anything, not just viruses.
Edited by bob1, 29 April 2009 - 04:51 PM.