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Characterizing mouse ES cells: AP antibody?

Started by viobio, Apr 29 2009 09:57 AM

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Poll: Characterization of mouse ES cells (3 member(s) have cast votes)

How do you determine the pluripotency of your mouse ES cells?

alk phos substrate (1 votes [20.00%])
Percentage of vote: 20.00%
alk phos antibody (1 votes [20.00%])
Percentage of vote: 20.00%
other antibody (1 votes [20.00%])
Percentage of vote: 20.00%
RT-PCR (1 votes [20.00%])
Percentage of vote: 20.00%
morphology of colony (0 votes [0.00%])
Percentage of vote: 0.00%
western (0 votes [0.00%])
Percentage of vote: 0.00%
flow cytometry (1 votes [20.00%])
Percentage of vote: 20.00%
Hoechst exclusion (0 votes [0.00%])
Percentage of vote: 0.00%
lectin, e.g. DBA (0 votes [0.00%])
Percentage of vote: 0.00%
other method (0 votes [0.00%])
Percentage of vote: 0.00%

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#1 viobio

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Posted 29 April 2009 - 09:57 AM

Hi,

Some of my ES colonies look differentiated. I want to quantify and maybe sort the undifferentiated cells.
I am looking for a surface marker that is known to be expressed specifically in undifferentiated mouse ES cells. Ideally, I want an antibody or other reagent I could use with a FACS sorter. I know there are several SSEA-1 antibodies, but I can't find alkaline phosphatase antibodies that recognize mouse. Have you used alk phos substrate kits with FACS, or does the fluorescent product fall off the cell?

Thanks for any help!
Post-postdoc