Some of my ES colonies look differentiated. I want to quantify and maybe sort the undifferentiated cells.
I am looking for a surface marker that is known to be expressed specifically in undifferentiated mouse ES cells. Ideally, I want an antibody or other reagent I could use with a FACS sorter. I know there are several SSEA-1 antibodies, but I can't find alkaline phosphatase antibodies that recognize mouse. Have you used alk phos substrate kits with FACS, or does the fluorescent product fall off the cell?
Thanks for any help!
Characterizing mouse ES cells: AP antibody?
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