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Characterizing mouse ES cells: AP antibody?


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Poll: Characterization of mouse ES cells (3 member(s) have cast votes)

How do you determine the pluripotency of your mouse ES cells?

  1. alk phos substrate (1 votes [20.00%])

    Percentage of vote: 20.00%

  2. alk phos antibody (1 votes [20.00%])

    Percentage of vote: 20.00%

  3. other antibody (1 votes [20.00%])

    Percentage of vote: 20.00%

  4. RT-PCR (1 votes [20.00%])

    Percentage of vote: 20.00%

  5. morphology of colony (0 votes [0.00%])

    Percentage of vote: 0.00%

  6. western (0 votes [0.00%])

    Percentage of vote: 0.00%

  7. flow cytometry (1 votes [20.00%])

    Percentage of vote: 20.00%

  8. Hoechst exclusion (0 votes [0.00%])

    Percentage of vote: 0.00%

  9. lectin, e.g. DBA (0 votes [0.00%])

    Percentage of vote: 0.00%

  10. other method (0 votes [0.00%])

    Percentage of vote: 0.00%

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#1 viobio

viobio

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Posted 29 April 2009 - 09:57 AM

Hi,

Some of my ES colonies look differentiated. I want to quantify and maybe sort the undifferentiated cells.
I am looking for a surface marker that is known to be expressed specifically in undifferentiated mouse ES cells. Ideally, I want an antibody or other reagent I could use with a FACS sorter. I know there are several SSEA-1 antibodies, but I can't find alkaline phosphatase antibodies that recognize mouse. Have you used alk phos substrate kits with FACS, or does the fluorescent product fall off the cell?

Thanks for any help!
Post-postdoc




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