30 replies to this topic

[perneseblue]

cotchy, on May 1 2009, 12:02 AM, said:

I havent tried this one so what happens when you put dry ice in an eppendorf tube?.

The lid pops off and an audible sound is heard.

cotchy, on May 1 2009, 12:02 AM, said:

And out of curiosity how would you do this?

1. To an empty eppendorf tube place one pellet of dry ice into the tube
2. Close the cap of the eppendorf tube.
3. Quickly place sealed tube in a warm place
4. Retreat to a safe distance.
5. Wait
Note: Beware of patrolling safety officer. Safety first. Wear safety glasses.

cotchy, on May 1 2009, 12:02 AM, said:

Do I just add some of the ice pellets to an eppendorf? Should i stand back pretty quickly after doing it?

Yes. You have a few minutes. Mostly it depends on the room temperature. Do note that your hands are warm. Use a rack to more the tube. Warm hands will sublime the carbon dioxide pellet.

cotchy, on May 1 2009, 12:02 AM, said:

(Of course i just want to find out what not to do, you know.... in case it happened to me by accident !!!, I wouldnt dream of putting dry ice in an eppendorf )

Most certainly. This is purely a hypothetical discussion, in the even that you should find dry ice in a sealed eppendorf. The more extreme version of this endeavour is to place liquid nitrogen into a closed eppendorf tube. The bang is louder, more dramatic and more dangerous. Real injury is possible.

[toejam]

perneseblue, on May 1 2009, 10:05 AM, said:

cotchy, on May 1 2009, 12:02 AM, said:

(Of course i just want to find out what not to do, you know.... in case it happened to me by accident !!!, I wouldnt dream of putting dry ice in an eppendorf )

Most certainly. This is purely a hypothetical discussion, in the even that you should find dry ice in a sealed eppendorf. The more extreme version of this endeavour is to place liquid nitrogen into a closed eppendorf tube. The bang is louder, more dramatic and more dangerous. Real injury is possible.

please do not try this at home, or should i say at the lab, unless you're qualified to...

[hobglobin]

toejam, on May 1 2009, 12:23 PM, said:

perneseblue, on May 1 2009, 10:05 AM, said:

cotchy, on May 1 2009, 12:02 AM, said:

(Of course i just want to find out what not to do, you know.... in case it happened to me by accident !!!, I wouldnt dream of putting dry ice in an eppendorf )

Most certainly. This is purely a hypothetical discussion, in the even that you should find dry ice in a sealed eppendorf. The more extreme version of this endeavour is to place liquid nitrogen into a closed eppendorf tube. The bang is louder, more dramatic and more dangerous. Real injury is possible.

please do not try this at home, or should i say at the lab, unless you're qualified to...

In my institute, once some PhD students throw a heap of dry ice (what's inside a parcel from any company sending biological material in dry ice) in a large puddle on the institutes parking area...nice effect the vapour and big dirty bubbles. But finally someone called the fire brigade...not so nice.  

[hanming86]

seeing bacterial colony on agar plate makes me happy.

it's a sign of life.  life is good and life goes on

[madrius1]

Pouring liquid nitrogen on the floor and watch the small droplets bounce of the wall as they hit it!

[Telomerase]

Watching baby mice in the animal room. That's why I get stuck in there twice as long as I need

[casandra]

yeah, like you don't wanna leave. Mine would be whenever I go to the microscope/imaging facility....the walls are lined with posters and enlarged pics of stained cells and tissues, some of  Nikon's Small World past winners....the brilliant colours hit you first and then the intricate designs......an art gallery right there....

and then you go thru these inevitable stages: first, awe and appreciation "nature is perfect, the guys from AT&T Bell Labs and Zeiss,  geniuses"; then the frustration "why are my images never this good"; and lastly, the art critic comes out: "enhanced, enhanced, touched -up, photoshopped, what the heck are those?"  

hope everyone's having a nice weekend....

casandra

Edited by casandra, 02 May 2009 - 05:29 AM.

[miRNA man]

Dipping my bacteria spreader in ethanol and then burning it off

[lab rat]

Filling out the last page of an old lab book (perfectly to the bottom, no overflow).

Filling out the first page of a new lab book.

Finding that someone brought a can of cinnamon buns to the breakroom and baked them in the communal toaster oven just before coffee break time.

[Danina]

Well, I suppose the eppendorf tube will explode due to increased pressure of CO2, yes?

What makes me happy in the lab? that's a really good question

I'm happy when I have to streak out some bacteria on LB agar/Amp. I am happy when taking out old media by suction from my LNCaP flask. Sound weird, but it feels great to know that I have changed the medium

[SS17]

seeing  well stocked pipette racks makes me happy when on the hood

[toejam]

printing double sided. makes me feel "environmentally friendly"

[Minnie Mouse]

toejam, on May 5 2009, 07:42 AM, said:

printing double sided. makes me feel "environmentally friendly"

same here  

[Adrian K]

Nobody is using the hood or thermal cycler...so i can use.

Edited by adrian kohsf, 07 June 2010 - 09:51 PM.

[laurequillo]

toejam, on Apr 30 2009, 03:59 PM, said:

putting dry ice in eppendorf tubes....

I agree...playing around with the dry ice...puting in a biker with water and soap. Or play with the liquid nitrogen, putting things inside...yeahh..the joy of science...