Genomic DNA contamination in RNA sample
Posted 29 April 2009 - 08:08 AM
I have some problems to perform a RT-PCR becouse I have in my RNA sample a genomic DNA contamination. I treated my RNA sample using different DNase enzyme, but it didn't work. And I cannot using PCR primers construct in two exons.
Do you have any suggestion about it?
Thanks in advance
Posted 29 April 2009 - 04:30 PM
Posted 01 May 2009 - 04:19 PM
Another thing you could do is to dilute more your cDNA sample. Sometimes dilutions of 500x or 1000x are fine to avoid DNA amplification.
Edited by dvddecarvalho, 01 May 2009 - 04:29 PM.