Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

selection of internal control gene


  • Please log in to reply
2 replies to this topic

#1 makiyo

makiyo

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 29 April 2009 - 07:45 AM

I am going to do qPCR. My target gene is expressed at a very low level. Can I use housekeeping genes? I was told that comparable CTs between target and control are preferred, but can't find any official saying about this.

Edited by makiyo, 29 April 2009 - 07:51 AM.


#2 Dr Teeth

Dr Teeth

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 221 posts
1
Neutral

Posted 29 April 2009 - 08:11 AM

I am going to do qPCR. My target gene is expressed at a very low level. Can I use housekeeping genes? I was told that comparable CTs between target and control are preferred, but can't find any official saying about this.


It is more important that your amplification efficiencies for both genes (target and control) are similar, rather than their absolute Ct values. Some of the housekeeping genes should be fine.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#3 scolix

scolix

    a student

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 314 posts
5
Neutral

Posted 02 May 2009 - 02:42 AM

I would suggest using more than 1 house keeping gene just so that the results donot mislead you.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.