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selection of internal control gene

Started by makiyo, Apr 29 2009 07:45 AM

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#1 makiyo

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Posted 29 April 2009 - 07:45 AM

I am going to do qPCR. My target gene is expressed at a very low level. Can I use housekeeping genes? I was told that comparable CTs between target and control are preferred, but can't find any official saying about this.

Edited by makiyo, 29 April 2009 - 07:51 AM.

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#2 Dr Teeth

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Posted 29 April 2009 - 08:11 AM

makiyo, on Apr 29 2009, 11:45 AM, said:

I am going to do qPCR. My target gene is expressed at a very low level. Can I use housekeeping genes? I was told that comparable CTs between target and control are preferred, but can't find any official saying about this.

It is more important that your amplification efficiencies for both genes (target and control) are similar, rather than their absolute Ct values. Some of the housekeeping genes should be fine.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley