4 replies to this topic

[Tanja N]

I would like to analyze bacterial diversity in contaminated soil. I' ve seen some papers in which authors tell that the sampling should be done from 0 - 0.5 m (surface to 0.5m of depth), and my question is how to take samples, with which tool?
Thanks in advance,
T

[gebirgsziege]

Depends on what and where you want to sample.....usually you use a soil corer or similar example

The depth is depending on you question.....sometimes the upper 10 cm are enoutgh and the next time you need to go down 1 m......

And sometimes you will wish you had dynamite good luck!

[Tanja N]

gebirgsziege, on Apr 29 2009, 05:15 PM, said:

Depends on what and where you want to sample.....usually you use a soil corer or similar example

The depth is depending on you question.....sometimes the upper 10 cm are enoutgh and the next time you need to go down 1 m......

And sometimes you will wish you had dynamite good luck!

Thanks! Hope that I will have good luck!

[GeorgeWolff]

If you really want to understand the microbial diversity - culture is not the way to go.

Sounds like you're most interested in the soil associated with "contamination."  Suggest you focus on that part.

[Tanja N]

GeorgeWolff, on May 2 2009, 11:03 PM, said:

If you really want to understand the microbial diversity - culture is not the way to go.]


Sounds like you're most interested in the soil associated with "contamination."  Suggest you focus on that part.

That actually is my idea, to do the metagenomic analysis of contaminated soil. Also I would like to try culture dependent approach, hopefully to isolate single strains .

I have analyzed literature regarding that particular type of contamination, and I couldn't find any info about the contaminants concentration & soil depth & bacterial diversity correlations.  
So, as "gebirgsziege" has said I guess I will have to go for trial and errors.

Edited by Tanja N, 11 May 2009 - 06:58 AM.