Posted 28 April 2009 - 11:02 AM
I am doing transfections for the first time and I have a question about lipofectamine 2000.
It says to change media after 4-6 hours. I have a total of 600ul solution in each well of my plate. So should I just aspirate the entire solution out and then replace it will my fresh media? Or should I add more media to the wells and not remove anything from the well?
Posted 02 May 2009 - 02:47 AM
Posted 05 May 2009 - 06:17 AM