5 replies to this topic

[DocFlow]

Has anybody on here any experience with and/or OK protocols for DNA extraction from buccal swabs or saliva, either manual extraction or automated with extractors like M48, magNA Pure etc? Any help would be appreciated.
What is a normal yield from such DNA extraction?

Thanks in advance.

[merlav]

Hi:  I had use BuccalAmp from Epicentre Biotechnologies is very very easy.  In general the yield from buccal samples is low, because of the constant sheading of the cells and the majority are dead.

[DocFlow]

merlav, on Apr 28 2009, 02:27 PM, said:

Hi:  I had use BuccalAmp from Epicentre Biotechnologies is very very easy.  In general the yield from buccal samples is low, because of the constant sheading of the cells and the majority are dead.

Thanks! I'll def check them out. In general, does that mean that the next step is a whole genome amplicication?

anybody had any experience with DNA from buccal swabs/mouth wash and a following SNP analysis? Is that even possible at all without an amplification step in between?

[AquaPlasmid]

I suggest you look into our AquaGenomic protocol. If extracting from mouthwash, you will get tons of DNA.

[nbhu]

Hi.
I actually found that using simple phenol chloroform extraction worked the best after treating with proteinase K to killany proteins. It depends how much buccal swab product there is, but if there is a fair bit, then i acheived yields of ~500ng/ul+

[merlav]

Phenol-chloroform produce dangerous wastes, I prefer kits that reduce this problems.