Calculating MOI for luciferase-transducted cells.
Posted 27 April 2009 - 12:18 PM
I need to measure MOI (multiplicity of infection) for a viral vector carrying a luciferase gene.
I will be infecting adherent cultures in 24 well plates.
I know that for GFP, I would just look at GFP fluorescence in a fluorescence microscope.
But how would I see the Luciferase-expressing cells?
I assume, I need to add a substrate, which will be taken by cells and they would luminescence.
Would the sensitivity of a fluorescent microscope be enough to see the signal?
Posted 27 April 2009 - 05:38 PM
Anyway, if you are infecting with a pure stock of the virus (i.e. not in conjunction with another virus), then just assess the MOI as you would normally. The GFP doesn't do anything special.
Posted 28 April 2009 - 04:50 AM