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Protocol for 16S rDNA real time PCR

Started by nee, Apr 27 2009 07:47 AM

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#1 nee

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Posted 27 April 2009 - 07:47 AM

Hi everyone...

I am very new in this field of molecular biology....I have been asked to detect bacteria in chicken ileum using the 16S rDNA real time PCR technique...Does anyone have a protocol for that? or any good paper?
plse help me...i'm near to tears!

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#2 chrisbelle

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Posted 27 April 2009 - 07:13 PM

nee, on Apr 27 2009, 11:47 PM, said:

Hi everyone...

I am very new in this field of molecular biology....I have been asked to detect bacteria in chicken ileum using the 16S rDNA real time PCR technique...Does anyone have a protocol for that? or any good paper?
plse help me...i'm near to tears! :wacko:

Hey there,
you can try to read papers like this: http://jmm.sgmjourna...stract/52/8/685
protocols are usuallly modified for your own samples and pcr reagents. good luck!!

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