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Protocol for 16S rDNA real time PCR


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#1 nee

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Posted 27 April 2009 - 07:47 AM

Hi everyone...

I am very new in this field of molecular biology....I have been asked to detect bacteria in chicken ileum using the 16S rDNA real time PCR technique...Does anyone have a protocol for that? or any good paper?
plse help me...i'm near to tears! :)

#2 chrisbelle

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Posted 27 April 2009 - 07:13 PM

Hi everyone...

I am very new in this field of molecular biology....I have been asked to detect bacteria in chicken ileum using the 16S rDNA real time PCR technique...Does anyone have a protocol for that? or any good paper?
plse help me...i'm near to tears! :wacko:


Hey there,
you can try to read papers like this: http://jmm.sgmjourna...stract/52/8/685
protocols are usuallly modified for your own samples and pcr reagents. good luck!!
Life sucks. Enjoy it while you can.

#3 nee

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Posted 28 April 2009 - 07:41 AM

Thank you so much for your help




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