Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Touchdown PCR


  • Please log in to reply
1 reply to this topic

#1 Kami23

Kami23

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 95 posts
0
Neutral

Posted 27 April 2009 - 07:37 AM

Hey all,

Just wondering if anyone has a pretty generic touchdown pcr protocol? doesnt have to be too specific :)

#2 gebirgsziege

gebirgsziege

    I like fungi

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 392 posts
25
Excellent

Posted 27 April 2009 - 11:31 PM

I usually use the following: 2x 65 C, 2 x 62C, 2 x 60C, 5 x 58 C, 20 x 56 C

works fine for me :wacko:
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.