8 replies to this topic

[sagar]

hi all

im trying to grow arabidopsis in solid agar media but the seeds donot grow .what can be other methods to make them grow ?
can anyone help?

[Signal]

sagar, on Apr 27 2009, 04:25 AM, said:

hi all

im trying to grow arabidopsis in solid agar media but the seeds donot grow .what can be other methods to make them grow ?
can anyone help?

What do you mean by solid agar media? is it 1/2MS containing agar? What is the germination %age of the seeds? How long do you keep the plates at 4 degrees for stratification?
I assume you are trying to grow mutants, right? You can grow some wild type seeds on the same media just to check in case if there is some problem with the media or with protocol.

Edited by Signal, 28 April 2009 - 05:35 AM.

[sagar]

Signal, on Apr 28 2009, 07:01 PM, said:

sagar, on Apr 27 2009, 04:25 AM, said:

hi all

im trying to grow arabidopsis in solid agar media but the seeds donot grow .what can be other methods to make them grow ?
can anyone help?

What do you mean by solid agar media? is it 1/2MS containing agar? What is the germination %age of the seeds? How long do you keep the plates at 4 degrees for stratification?
I assume you are trying to grow mutants, right? You can grow some wild type seeds on the same media just to check in case if there is some problem with the media or with protocol.

hi

Actually i had kept the arabisopsis seeds at 4C for 2 days and then i got germination of only a few arabidopsis seeds-4% very less regeneracy.
I am not certain whether keeping the seeds in constant motion is better than just keeping them in static condition at 4C.

Any suggestions

[toejam]

i suggest you to look for papers about breaking seed dormancy in arabidopsis, or to make a quick germination assay. good luck

[JayBest]

You can find out at seedbiology for your doubt and query... I think it will help....





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[bullfrog]

perhaps the seeds were not stored properly...
In addition to stratefication treatment, you can treat seeds with 10 uM GA

[fighter]

another small question..is there any problem if I keep the seeds on plate in 4C bit longer time..like over 10 days and transfer them to culture room?? Would you expect any effect on germination rate??

[toejam]

haven't read these reviews myself but i guess they'll be useful for you.

http://rspb.royalsocietypublishing.org/con.../1673/3561.full

http://aob.oxfordjou.../full/102/5/877


NB: i'm not working on arabidopsis so i'm unable to provide reliable experimental information on the topic.

[fighter]

Thank you toejam. Hope these reviews will give me the necessary information..

toejam, on Nov 5 2009, 06:09 AM, said:

haven't read these reviews myself but i guess they'll be useful for you.

http://rspb.royalsocietypublishing.org/con.../1673/3561.full

http://aob.oxfordjou.../full/102/5/877


NB: i'm not working on arabidopsis so i'm unable to provide reliable experimental information on the topic.

Edited by fighter, 07 November 2009 - 12:30 AM.