Looking for new endogenous control for WB
Posted 27 April 2009 - 02:32 AM
I had problems with stripping my mebrane so I decided to cut it instead. But now I have a problem with the endogenous control. One of my protein of intrest is just in the same range as beta-acin. So what could I use instead. I thought of Tubulin but this is also running in the same range of a protein of intrest. Ist there somehing smaller than 30 kDa or larger than 60 kDa i could use?
Thank you for your attention.
Posted 27 April 2009 - 04:49 AM
Edited by yobou, 27 April 2009 - 04:50 AM.
Posted 27 April 2009 - 05:58 AM
Use RNA pol II (~220 kD).
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
Posted 27 April 2009 - 06:13 AM
Stripping is not working. I use the same protocol and the same solutions as the others do in my lab but somehow my proteins are always gone. I transfered longer, used different membranes, but it's as it does not like me.
Even when I am doing it with together with someone else, they disapear.
is this published somewhere? And do you know also something for the cytoplasmic fraction?
Edited by Sumpf, 27 April 2009 - 06:15 AM.