1)I've a 420 bp PCR fragment. On restriction enzyme digestion 2 fragments are produced- 386bp and 34 bp..
I tried running the RFLP products and the undigested PCR product (control) on 2 % agarose but i've failed to obtain a good resolution..Can any1 suggest wht percentage of agarose wud be appropriate to obtain a good resolution.
2)Also when i load my RFLP samples it seems to run unevenly ( the samples form an arc) .Can this be due to excess sample loading.. I checked the buffer level..the gel is always immersed in the buffer (TBE). The voltage that i apply is 120. and yes the arc is obtained when i load the sampl in another appratus too.Plz help...
Edited by Shruti B, 26 April 2009 - 04:25 AM.