Agarose gel electrophoresis problems
Posted 24 April 2009 - 03:39 AM
Posted 24 April 2009 - 04:15 AM
it looks to me like your ladder is degraded.. how old is it? also do you change tips every time you dip in (EtBr will degrade the DNA)? Do you aliquot it first (this protects against the effects of freeze-thaw)?
Posted 24 April 2009 - 04:49 AM
Are you sure you're casting the gels in the buffer you're running them in, and not in water? Are you sure the buffer is the right strength?
Do you have another power supply you can try?
Is the power supply set to constant voltage or constant current?
Do you have another gel electrophoresis chamber you can try?
Posted 24 April 2009 - 07:54 AM
Posted 24 April 2009 - 08:39 AM
It might be a density problem related to this or the agarose not being melted properly.
Posted 24 April 2009 - 10:35 AM
genius does what it must
i do what i get paid to do
Posted 24 April 2009 - 09:00 PM
Posted 25 April 2009 - 03:38 PM
Please stirr after every few min of boiling youer agarose and they will disappear.
Best of luck.
Edited by nirajm, 25 April 2009 - 03:38 PM.