Hi everyone,
I ordered a tetramer specific for my peptide of interest on the H2Kd allele from NIH and I'll be using it to quantify peptide specific CD8s in Balb/c mice post-infection. Would I be able to use this tetramer to analyze CD8s in B6 mice or do I have to order a new tetramer?
This is my first year working in an immuno lab and my knowledge on the subject is fairly limited. Ahy help would appreciated. Thanks!
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Posted 23 April 2009 - 04:10 PM
It's highly likely that you'll be fine using the same tetramer, but the only way to be 100% certain is to try and find out. ... I'd bet $ that you'll be fine, though. The genetic differences between the two mouse lines are incredibly small.
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