Edited by deepbluedna, 23 April 2009 - 06:55 AM.
truncation of protein expression
Posted 23 April 2009 - 06:54 AM
Posted 23 April 2009 - 07:58 PM
I am not sure what you mean by this but if it is a Nter truncation, it could be because of some protease. U can try some protease inhibitors during purification and see if that helps. However, in one of the proteins that we have cloned in the lab, truncation is seen and proteases don't help.
the protein is truncated from one amino acid after the 1st methionine to another 14 amino acids following.