GFP reporter gene
Posted 23 April 2009 - 06:50 AM
I will like to create a fusion gene between GFP reporter gene and GPCR gene is it possible ?
and it is possible how can I do it if I want it to express in animal?
Posted 23 April 2009 - 08:18 AM
One of them is to use a vector with multiple cloning site before or after GFP (be careful to be in frame and to get rid of the stop codon if you put your gene before the GFP), or you could PCR your gene of interest and insert the PCR product in a plasmid such as pAcGFP1-N In-Fusion™ Ready Vector from clontech. Have a look on their website. This plasmid can also be expressed in mammalian cells.
Posted 23 April 2009 - 12:07 PM
Posted 24 April 2009 - 12:43 AM
Then you should post it somewhere else, so we could help you.