polymerase for BSP
Posted 23 April 2009 - 05:20 AM
I red on this forum that polymerase for bisulfite PCR shuld be non proofreading. I want to ask is it true and if it is, why?
Which polymerase do you recommend?
Posted 23 April 2009 - 02:31 PM
I believe the proof reading polymerase would either stall or try to correct the mismatches arising from this base change in the first few cycles. This could potentially overestimate or underestimate the methylation calls as you are trying to detect a C or T for DNA methylation.
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
Posted 08 May 2009 - 05:56 PM