Posted 13 June 2011 - 05:17 AM
do you have experience with TEER measurements on HaCaT cells? I measured it, but the values were the same for blanks as well (transwell without HaCaT cells had the same TEER value as Transwell with HaCaT cells). Do you know what could be the reason?
I was thinking that HaCaT cells were not grown as monolayer and therefore TEER could not be measured - because I think that TEER is possible to measure only on monolayer. Do you have explanation?
Posted 25 August 2011 - 12:32 PM
Hi all, a colleauge of mine has recently started to work with HACAT cells.
He bought them in a while ago from CLS (cell line company) at a low passage number and passaged them a few times and then froze them down in the - 80C (70 % DMEM, 20 % serum, 10 % DMSO) and then after a few days places them in liquid nitrogen
When he brings them up from the liquid nitrogen (in the proper recommended media - DMEM w/ 10 % serum ) for use the viabilty of the cells is always very low, around 2 - 5 % and thus it takes 2 to 3 weeks to get a T 25 confluent.
Does anybody have any suggestions as to what is causing the low viability of the cells or has anybody had the same problems and managed to overcome them?
A US company sells the same cells, here is the link [link]http://addexbio.com/...tdetail?pid=117[/link]. Check that out to see if they also come up with culture condition for this cell line.
- chowyc2 likes this
Posted 25 August 2011 - 10:55 PM
Posted 05 December 2011 - 02:46 PM