Possible to lose a pGEM insert ?
Posted 22 April 2009 - 10:59 PM
i have a quick question, can an insert (about 500 bp) "fall out" of pGEM once ligated into it ?
am i stupid for thinking it can be lost !
Posted 26 April 2009 - 02:25 PM
Or you could you cut the plasmid with EcoRI, for verify the integrity of your construction.
Edited by Felipillo, 26 April 2009 - 02:26 PM.
Posted 03 May 2009 - 01:51 AM