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Increase DNA yield with glycogen


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#1 anonymous

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Posted 13 November 2001 - 10:00 PM

I am working with very small amount human tissue. Is there anyone who had the experience of using glycogen with 100% ethanol in pheno/chloroform DNA extraction protocol ? Does it realy work to increase DNA yield much?

#2 anonymous

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Posted 13 November 2001 - 10:00 PM

The glycogen is just there as a co-precipitant to ensure that you do not lose your pellet and won't do anything to your 260/280 readings. If you are really worried about this step then Ambion do a product called glyco blue which makes it virtually impossible to lose the pellet.

#3 anonymous

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Posted 03 December 2001 - 10:00 PM

I have used glycogen ( about 2 micrograms ) for precipitating In vitro transcribed RNA.sankar




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