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cloning


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#1 KAMALAKER

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Posted 22 April 2009 - 09:01 AM

How to calculate the insert and linear plsmid concentration for ligation

How much restriction enzymes need to be added for 1 micro gram of DNA IS it varies between plasmid DNA, and plant genomic DNA.


Please give me as much as possible explanation for these things.

#2 bob1

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Posted 22 April 2009 - 04:19 PM

Run your samples through a spectrophotometer and look at the absorbance at 260 and 280 nm - go to the protocols page on this site, under molecular biology there are a heap of links that will tell you all you need to know.




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