How to calculate the insert and linear plsmid concentration for ligation
How much restriction enzymes need to be added for 1 micro gram of DNA IS it varies between plasmid DNA, and plant genomic DNA.
Please give me as much as possible explanation for these things.
Submit your paper to J Biol Methods today!
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 5,913 posts
Thelymitra pulchella
427
Excellent
Excellent
Posted 22 April 2009 - 04:19 PM
Run your samples through a spectrophotometer and look at the absorbance at 260 and 280 nm - go to the protocols page on this site, under molecular biology there are a heap of links that will tell you all you need to know.
