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PFGE gel


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#1 tana_jung

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Posted 22 April 2009 - 08:33 AM

I'm running Pulsed field gel electrophoresis for P.aeruginosa...
I use 1% pulsed field certified gel for seperating..

Can I reuse this gel??
And if I can reuse how many times should I reuse??

Please, advise me!!
thank you very much for your advice :lol:


-Cherman-

#2 perneseblue

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Posted 22 April 2009 - 08:40 AM

I'm running Pulsed field gel electrophoresis for P.aeruginosa...
I use 1% pulsed field certified gel for seperating..

Can I reuse this gel??
And if I can reuse how many times should I reuse??

Please, advise me!!
thank you very much for your advice :lol:


-Cherman-


I would be happy to reuse the gel for normal electrophoresis (with adjustments to the gel buffer concentration). But I would not reuse the gel for PFGE. You can't afford the same level of DNA degradation with intact chromosomes compared to plasmid DNA. It is easier to smear.
May your PCR products be long, your protocols short and your boss on holiday

#3 tana_jung

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Posted 22 April 2009 - 08:53 AM

I'm running Pulsed field gel electrophoresis for P.aeruginosa...
I use 1% pulsed field certified gel for seperating..

Can I reuse this gel??
And if I can reuse how many times should I reuse??

Please, advise me!!
thank you very much for your advice :D


-Cherman-


I would be happy to reuse the gel for normal electrophoresis (with adjustments to the gel buffer concentration). But I would not reuse the gel for PFGE. You can't afford the same level of DNA degradation with intact chromosomes compared to plasmid DNA. It is easier to smear.



thank you very much for your good suggestion.. :D




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