1 reply to this topic

[StevenRed]

Hello everyone
Just want to ask a basic question for co-immunoprecipitation

I know Co-IP can pull the known protein and its entire complex out of the solution by binding the antibody with that protein...
But if I want to identify the unknown members ithat I pull out in the complex, waht can I do?

[Dr Teeth]

StevenRed, on Apr 21 2009, 01:15 PM, said:

Hello everyone
Just want to ask a basic question for co-immunoprecipitation

I know Co-IP can pull the known protein and its entire complex out of the solution by binding the antibody with that protein...
But if I want to identify the unknown members ithat I pull out in the complex, waht can I do?

There is no easy way to identify interacting proteins (of unknown origin) with a co-IP. If you have a suspected interactor, you can do a Western after co-IP, though if you don't have an antibody already for your suspected protein, this could become quite costly.  If the proteins are completely unknown, you would need to do mass spec.