i'm trying to measure h2o2 using the scopoletin method. i'm really having issues with it has any one any idea how to do this method or could PM me the following paper which may help. The scopoletin assay for hydrogen peroxide. A review and a better method. Corbett JT. : J Biochem Biophys Methods. 1989 Jun;18(4):297-307. OR Evaluation of the horseradish peroxidase-scopoletin method for the measurement of hydrogen peroxide formation in biological systems.A. Boveris, E. Martino and A. O. M. Stoppani. Anal Biochem. 1977 May 15;80(1):145-58
i cant access it from my institution.
Edited by proteinz, 21 April 2009 - 08:56 AM.