Posted 21 April 2009 - 02:17 AM
Posted 21 April 2009 - 08:13 AM
genius does what it must
i do what i get paid to do
Posted 21 April 2009 - 01:58 PM
Posted 22 April 2009 - 01:52 AM
Treat your sample with the storage buffer and a suitable control. If the protein precipitates, a pellet will be visible upon centrifugation. However, the pellet will be discernible only if the concentration of your protein is high enough to produce a large one. In case you do not see any pellet, run SDS-PAGE with the supernatant along with the control and comapre the intensity of the bands. If you have a functional assay for your protein then try it with the supernatant. Note: do not forget to inlcude a good control; this is a compatative anlaysis.
thx guys. My protein is an enzyme so I am going to use enzyme assay for testing