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PCR and Cultures


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#1 jcl

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Posted 26 January 2002 - 01:48 AM

I am looking for a way to extract DNA out of my algae cultures (it is a Bold mineral medium). I know that some components in the culture media can inhibit a PCR reaction. Has anybody good suggestions or even experiences with this kind of problem? Many thanks.

#2 Cthulhu

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Posted 26 January 2002 - 06:23 AM

Have you tried it and it didn't work? I'm thinking these inhibitors would disappear in the DNA preparation.




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