On the gel, there was no 18S band while 28S band was present. I ran the gel again thinking I made the gel incorrectly, but the results were the same. Doesn't seem like a degradation problem since 28S band is intact. Anyone have any idea as to why this is occurring?
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sihyunie
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Posted 20 April 2009 - 05:35 PM
In order to check RNA purity and integrity before northern blot, I ran the samples on a 1% agarose gel.
On the gel, there was no 18S band while 28S band was present. I ran the gel again thinking I made the gel incorrectly, but the results were the same. Doesn't seem like a degradation problem since 28S band is intact. Anyone have any idea as to why this is occurring?
On the gel, there was no 18S band while 28S band was present. I ran the gel again thinking I made the gel incorrectly, but the results were the same. Doesn't seem like a degradation problem since 28S band is intact. Anyone have any idea as to why this is occurring?
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dvddecarvalho
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Posted 20 April 2009 - 06:40 PM
You did a formamide/formaldehyde denaturing gel??
