4 replies to this topic

[roshanbernard]

hi,
i have a vector which has Ap and ery resistance gene...and i have got this vector from the author,who has transformed the plasmid into L.Lactis and sent me....now when i want to culture it using M17 media...which antibiotic i should add to the media in order to extract the plasmid.....

[T C]

Shouldn't it be Ap or ery or both?

roshanbernard, on Apr 21 2009, 08:00 AM, said:

hi,
i have a vector which has Ap and ery resistance gene...and i have got this vector from the author,who has transformed the plasmid into L.Lactis and sent me....now when i want to culture it using M17 media...which antibiotic i should add to the media in order to extract the plasmid.....

[little mouse]

I would say ampicillin for bacteria, and erythromycin for eucaryote cells.

[aimikins]

Missele, why wouldn't you use Er for bacteria? I would think it would be more effective and stable than Ap?

[roshanbernard]

thanks a lot guys for the reply...i will use ery for the mactis culture i.e plamid selection and when i transform it into the e coli i will use ery and amp...thanks once again for the help.