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about antibiotic addition


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4 replies to this topic

#1 roshanbernard

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Posted 20 April 2009 - 05:30 PM

hi,
i have a vector which has Ap and ery resistance gene...and i have got this vector from the author,who has transformed the plasmid into L.Lactis and sent me....now when i want to culture it using M17 media...which antibiotic i should add to the media in order to extract the plasmid.....

#2 T C

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Posted 20 April 2009 - 08:24 PM

Shouldn't it be Ap or ery or both?


hi,
i have a vector which has Ap and ery resistance gene...and i have got this vector from the author,who has transformed the plasmid into L.Lactis and sent me....now when i want to culture it using M17 media...which antibiotic i should add to the media in order to extract the plasmid.....



#3 little mouse

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Posted 21 April 2009 - 02:01 AM

I would say ampicillin for bacteria, and erythromycin for eucaryote cells.

#4 aimikins

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Posted 21 April 2009 - 09:16 AM

Missele, why wouldn't you use Er for bacteria? I would think it would be more effective and stable than Ap?
"it is a miracle that curiosity survives formal education" -A.E.

#5 roshanbernard

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Posted 21 April 2009 - 03:59 PM

thanks a lot guys for the reply...i will use ery for the mactis culture i.e plamid selection and when i transform it into the e coli i will use ery and amp...thanks once again for the help.




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