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what is the activity of TAQ at 60 centigrade?


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#1 zgjabcxyz

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Posted 20 April 2009 - 09:00 AM

hi there,
I want to decrease my extension temperature to 60 centigrade.how many bps per second does TAQ do extension?
thank you in addvance.

#2 mdfenko

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Posted 20 April 2009 - 10:13 AM

beckman-coulter recommends an extension temperature of 60C for 4 minutes for cycle sequencing with the genomelab dtcs kit (they use taq). this can generate up to about 900-1000 bases (not necessarily all readable).

this is not to say that taq doesn't work faster (in fact, i'm sure that it does work faster) but this works out to ~250 bases/minute or ~4 bases/second.
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#3 zgjabcxyz

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Posted 21 April 2009 - 12:02 AM

beckman-coulter recommends an extension temperature of 60C for 4 minutes for cycle sequencing with the genomelab dtcs kit (they use taq). this can generate up to about 900-1000 bases (not necessarily all readable).

this is not to say that taq doesn't work faster (in fact, i'm sure that it does work faster) but this works out to ~250 bases/minute or ~4 bases/second.


THANK YOU VERY MUCH.
My target sequence has high GC% in middle and high AT% in the terminal.




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