Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

coating tissue culture lates with fibronectin


  • Please log in to reply
1 reply to this topic

#1 youyou

youyou

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 20 April 2009 - 06:14 AM

Hi everyone

could someone advice me on how to coat my tissue culture plates with fibronectin? the negative and positive controls? and do i have to culture my cells in serum free medium in this condition?
Thanx

Edited by youyou, 20 April 2009 - 06:16 AM.


#2 aimikins

aimikins

    Sunflower

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 117 posts
2
Neutral

Posted 20 April 2009 - 02:40 PM

I can't answer the SFM, but as for coating I used to do that pretty often.

make fibronectin at 1mg/mL in PBS. it will be clumpy, so assume your 'real' concentration is a bit lower. this is OK, because mostly you are just using an excess amount and you'll get good coverage.

vortex the holy living daylights out of the tube.

pipet an appropriate volume into your well/dish/whatever.

minimum time is 1 hour at 37C. I like to leave it overnight or 1/2 day if it's a critical step. this is the method we used when studying skin keratinocytes at my last job.
"it is a miracle that curiosity survives formal education" -A.E.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.