Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

supplement MEM with phenol-red???


  • Please log in to reply
3 replies to this topic

#1 shs

shs

    member

  • Active Members
  • Pip
  • 19 posts
2
Neutral

Posted 19 April 2009 - 07:22 AM

Dears
I just have received MEM media but I superised when I see its color is colorless that means I have to supplement it with phenol red....it is 100ml (1000X) so how much of phenol red should be addad and should I resterilize the media after adding phenol red and recheck the pH????

Any help??
Science is What We Need To Understan The Life

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,522 posts
371
Excellent

Posted 19 April 2009 - 04:20 PM

You don't have to add phenol red. Phenol red is just a pH indicator that has the minor advantage of making it easier to tell you when to split your cells. However, you should never rely on phenol red for this as the pH of the solution can be affected by many things including the %CO2 in your incubator, presence of a bacterial of fungal contamination. What you should be doing is inspecting your cells daily and looking at how frequently you passage your cells and growth rates etc.

If you do decide to supplement, and you definitely don't have to: a 100X solution is diluted 1 part in 100, i.e. 1 ml with 99ml diluent. If both solutions are sterile and you are careful with your technique, you can add one to the other, as you would for FCS/FBS into medium. If you need to sterilise, you will have to filter through a 0.2 um filter.

#3 shs

shs

    member

  • Active Members
  • Pip
  • 19 posts
2
Neutral

Posted 29 April 2009 - 09:27 PM

Thank you somuch
Science is What We Need To Understan The Life

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,522 posts
371
Excellent

Posted 30 April 2009 - 04:37 PM

I should also add - phenol red is a weak estrogen analogue, if your cells express estrogen receptor (most breast cell lines do, but it isn't restricted to them), then you shouldn't use phenol red in the culture.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.