supplement MEM with phenol-red???
Posted 19 April 2009 - 07:22 AM
I just have received MEM media but I superised when I see its color is colorless that means I have to supplement it with phenol red....it is 100ml (1000X) so how much of phenol red should be addad and should I resterilize the media after adding phenol red and recheck the pH????
Posted 19 April 2009 - 04:20 PM
If you do decide to supplement, and you definitely don't have to: a 100X solution is diluted 1 part in 100, i.e. 1 ml with 99ml diluent. If both solutions are sterile and you are careful with your technique, you can add one to the other, as you would for FCS/FBS into medium. If you need to sterilise, you will have to filter through a 0.2 um filter.
Posted 29 April 2009 - 09:27 PM
Posted 30 April 2009 - 04:37 PM