and what Cladosporium I am using? no idea, they just told me that one sample is Cladosporium and the other Penicilium.
And the roux bottles? thats what they gave me.
Maybe you then should just ask your supervisor about some more details, they should know about the sporulation problem. Cladosporium needs app. 1 - 2 weeks to sporulate well.
A personal opinion to add: "they just told me"
Every time I heard this sentence in the last years it was after some kind of (nearly) catastrophic event in the lab
So please: if somebody gives me cultures never start working with them without knowing some basic facts.....keep in mind every micro-organism we do not know must be treated as if it is a potential danger for us and our environment! Even if Cladosporium and Penicillium are harmless (HG1), they have a very high allergenic potential. So think about what you are working with before going to the bench! This usually saves you a lot of trouble and work!!! [End personal opininon]
Edited by gebirgsziege, 20 April 2009 - 10:40 PM.