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How to concentrate lenti or retroviral particules


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3 replies to this topic

#1 dvddecarvalho

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Posted 17 April 2009 - 04:55 AM

Dear friends,
I know that ultracentrifugation protocol is used to concentrate lent or retroviral particules. But this week I heard about a protocol using polyethylene glycol. Anybody here knows such protocol or any other?

Thanks,
Dvd

#2 gfischer

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Posted 17 April 2009 - 09:25 AM

Dear friends,
I know that ultracentrifugation protocol is used to concentrate lent or retroviral particules. But this week I heard about a protocol using polyethylene glycol. Anybody here knows such protocol or any other?

Thanks,
Dvd


There's also a method of concentration by anion exchange chromatography. I've never tried it, but if ultracentrifugation isn't an option, it might be worth a shot. Check out:

Gene Therapy (2002) 9, 1708-1714
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#3 Functional Screens

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Posted 18 April 2009 - 09:48 PM

Ultracentrifugation is good enough, PEG is not necessary.
The recovery rate is low with other methods.

#4 scolix

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Posted 03 May 2009 - 02:07 AM

I know that ultracentrifugation is the most common ways for concentrating lenti particles. Many labs use it for invivo stuff too. try and compare other techniques.




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