3 replies to this topic

[dvddecarvalho]

Dear friends,
I know that ultracentrifugation protocol is used to concentrate lent or retroviral particules. But this week I heard about a protocol using polyethylene glycol. Anybody here knows such protocol or any other?

Thanks,
Dvd

[gfischer]

dvddecarvalho, on Apr 17 2009, 07:55 AM, said:

Dear friends,
I know that ultracentrifugation protocol is used to concentrate lent or retroviral particules. But this week I heard about a protocol using polyethylene glycol. Anybody here knows such protocol or any other?

Thanks,
Dvd

There's also a method of concentration by anion exchange chromatography.  I've never tried it, but if ultracentrifugation isn't an option, it might be worth a shot.  Check out:

Gene Therapy (2002) 9, 1708-1714

[Functional Screens]

Ultracentrifugation is good enough, PEG is not necessary.
The recovery rate is low with other methods.

[scolix]

I know that ultracentrifugation is the most common ways for concentrating lenti particles. Many labs use it for invivo stuff too. try and compare other techniques.