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[dkunde]

Hi all

A fairly simple question I suppose but I am new to this.

What is the definition of a passage for a suspension cell line? For adherent cells it would be fairly straight forward ie everytime you trypsinize and reseed a new flask, but what about suspension cells ( in my case THP-1). Is it everytime you reseed a new flask, everytime you remove say 2/3 volume and add fresh media, everytime you add fresh media or all of the above?

Thanks in advance

Dale

[bob1]

It is every time you remove some of the cells and add fresh media.  Ideally, you would be looking at population doubling times while you are doing this, so as to have a better idea of when to passage.