Hi all,
I'm looking for a good protocol for Beta-Galactosidase Activity Assays that can be adapted to a 96-well plate format. Also, I need to use CPRG as the substrate, I bought it from Roche and don't know how to prepare it (in which solution and concentration?).
Thanks for the help!!!
Anahi
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3 replies to this topic
#2
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an elder
Posted 17 April 2009 - 08:30 AM
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
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member
Posted 17 April 2009 - 09:49 AM
[quote name='mdfenko' date='Apr 17 2009, 12:30 PM' post='22102']
you can adapt this assay to a 96 well plate:
[url="http://www.worthington-biochem.com/BG/assay.html"]beta galactosidase assay (worthington)[/url]
[/quot
Unfortunately, that protocol won't be useful, it doesn't guide me on how to prepare the substrate CPRG but also I'm doing this liquid Beta Gal assay to assess LacZ activation in a Y2H strain.
Thanks anyway,
you can adapt this assay to a 96 well plate:
[url="http://www.worthington-biochem.com/BG/assay.html"]beta galactosidase assay (worthington)[/url]
[/quot
Unfortunately, that protocol won't be useful, it doesn't guide me on how to prepare the substrate CPRG but also I'm doing this liquid Beta Gal assay to assess LacZ activation in a Y2H strain.
Thanks anyway,
#4
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Veteran
Posted 17 April 2009 - 04:23 PM
anahi,
check this out: Groth et al (1998) Anal Biochem 258: 141-3
http://www.sciencedirect.com/science?_ob=A...dd59664eb5334e2
check this out: Groth et al (1998) Anal Biochem 258: 141-3
http://www.sciencedirect.com/science?_ob=A...dd59664eb5334e2
